Microbial Exploitation of Feather Wastes for Sustainable Production of Keratinase and Collagenase Enzymes by Didymella keratinophila AUMC 15399 in Submerged Fermentation

A distinctive isolate was discovered and visually recognized as a member of the genus Didymella during routine examination Coelomycetes isolated from diverse fruit juices. Based on sequencing internal transcribed spacer (ITS), fungus identified keratinophila since it showed 100% identity to type strain. The strain thrived produced keratinase collagenase enzymes by hydrolyzing native chicken feathers in submerged fermentation (SmF). After 10 days at 30 °C, pH 9 using sodium nitrate nitrogen supply highest activity 8780 ± 620 U/mL/min, while 6 beef extract maximum 11,230 1290 U/mL/min. partially-purified enzyme worked best 7.0 45 exhibiting specific 44,903 1555 U/mg protein. excellent 6.0 35 generating 15,753 110 enzyme-specific activity. Mn2+ K+ were most efficient inhibitors keratinases collagenase, respectively. Both EDTA metal ions significantly decreased collagenase. This report workable supplier derived feathers, offering reliable way exploit manage these wastes for obtaining high-value products.